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Culture and Transfection of Zebrafish Primary Cells
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Culture and Transfection of Zebrafish Primary Cells

Culture and Transfection of Zebrafish Primary Cells

11,950 Views

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08:51 min

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August 17, 2018

DOI:

10.3791/57872-v

DOI:
10.3791/57872-v

•

08:51 min
August 17, 2018

•

13 Views
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1Division of Cellular and Molecular Neurobiology, Zoological Institute,Braunschweig University of Technology, 2Department of Biotechnology, Institute of Biochemistry, Biotechnology and Bioinformatics,Braunschweig University of Technology

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We present an efficient and easy-to-use protocol for preparing primary cell cultures of zebrafish embryos for transfection and live cell imaging as well as a protocol to prepare primary cells from adult zebrafish brain.

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Russo, G., Lehne, F., Pose Méndez, S. M., Dübel, S., Köster, R. W., Sassen, W. A. Culture and Transfection of Zebrafish Primary Cells. J. Vis. Exp. (138), e57872, doi:10.3791/57872 (2018).

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