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在利什曼原虫、人体细胞和小鼠睾丸中的 Polysome 分析
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Biochemistry
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JoVE Journal Biochemistry
Polysome Profiling in Leishmania, Human Cells and Mouse Testis
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在利什曼原虫、人体细胞和小鼠睾丸中的 Polysome 分析

DOI:
10.3791/57600-v

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14:32 min

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April 08, 2018

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, , , , , , , , ,
1Department of Cell Biology and Biochemistry,Texas Tech University Health Sciences Center, 2Department of Biological Sciences,Texas Tech University, 3CISER (Center for the Integration of STEM Education & Research),Texas Tech University

Chapters

  • 00:00Title
  • 01:24Lysate Preparation
  • 03:51Cytoplamic Lysate Preparation from Cultured Human Cells
  • 05:15Cytoplasmic Lysate Preparation from Mouse Testis
  • 06:53Sucrose Gradient Preparation and Ultracentrifugation
  • 09:21Polysome Fractionation and Sample Collection
  • 11:45Analysis of the Fractions
  • 12:27Results
  • 14:14Conclusions

Summary

Automatic Translation

Automatic Translation

polysome 分析技术的总体目标是分析个体基因或转录基因在蛋白质合成过程中的平移活动。该方法对健康和多人疾病的蛋白质合成调控、翻译活化和抑制的研究具有重要意义。

Tags

Polysome ProfilingMRNA TranslationProtein Synthesis RegulationLeishmaniaHuman CellsMouse TestisSucrose GradientUltracentrifugationRibosome FractionationCytoplasmic LysateCycloheximideCell CulturePBS WashCell Counting

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