Presented here is a phosphoproteomic approach, namely stop and go extraction tip based phosphoproteomic, which provides high-throughput and deep coverage of Arabidopsis phosphoproteome. This approach delineates the overview of osmotic stress signaling in Arabidopsis.
Hsu, C., Tsai, C., Tao, W. A., Wang, P. Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis. J. Vis. Exp. (160), e61489, doi:10.3791/61489 (2020).