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Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis
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Biochemistry
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JoVE Journal Biochemistry
Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

DOI:
10.3791/61489-v

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05:47 min

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June 25, 2020

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, , ,
1Department of Plant Biology,Carnegie Institute for Science, 2Graduate School of Pharmaceutical Sciences,Kyoto University, 3Department of Biochemistry,Purdue University, 4Department of Chemistry,Purdue University, 5Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences

Chapters

  • 00:04Introduction
  • 00:33Immobilized Metal Ion Affinity Chromatography (IMAC) and High pH Reversed-Phase (Hp-RP) Stage Tip Preparation
  • 01:10IMAC Stage Tip Phosphopeptide Enrichment
  • 02:44Hp-RP C18 Stage Tip Phosphopeptide Fractionation
  • 03:37Results: Representative Osmotic Stress Signaling in Arabidopsis
  • 05:05Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a phosphoproteomic approach, namely stop and go extraction tip based phosphoproteomic, which provides high-throughput and deep coverage of Arabidopsis phosphoproteome. This approach delineates the overview of osmotic stress signaling in Arabidopsis.

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Keywords: PhosphoproteomicsOsmotic StressArabidopsisIMACPhosphopeptide EnrichmentHigh PH Reverse Phase Fractionation

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