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Biochemistry
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JoVE Journal Biochemistry
Polysome Profiling in Leishmania, Human Cells and Mouse Testis
 

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Article DOI: 10.3791/57600-v • 14:33 min • April 8th, 2018
April 8th, 2018 •
2, 1, 1, 2,3, 1,3, 1, 1, 1, 2, 1
1Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, 2Department of Biological Sciences, Texas Tech University, 3CISER (Center for the Integration of STEM Education & Research), Texas Tech University

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The overall goal of polysome profiling technique is analysis of translational activity of individual mRNAs or transcriptome mRNAs during protein synthesis. The method is important for studies of protein synthesis regulation, translation activation and repression in health and multiple human diseases.

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Polysome Profiling MRNA Engagement Translation Protein Synthesis Regulation Translation Activation Translation Repression Polysomal Fractionation Sucrose Gradient Ultracentrifugation Sample Collection Analysis Of Fractions Lysis Buffer Sucrose Gradient Maker UV Detector Leishmania Major Human Cells Mouse Testis
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