Journal
/
Biochemistry
/
Polysome perfilado en Leishmania, las células humanas y testículo de ratón
JoVE Journal
Biochemistry
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Polysome Profiling in Leishmania, Human Cells and Mouse Testis
Please note that all translations are automatically generated. Click here for the English version.

Polysome perfilado en Leishmania, las células humanas y testículo de ratón

17,248 Views

•

14:32 min

•

April 08, 2018

DOI:

10.3791/57600-v

DOI:
10.3791/57600-v

•

14:32 min
April 08, 2018

•

6 Views
, , , , , , , , ,
1Department of Cell Biology and Biochemistry,Texas Tech University Health Sciences Center, 2Department of Biological Sciences,Texas Tech University, 3CISER (Center for the Integration of STEM Education & Research),Texas Tech University

Tags

Polysome ProfilingMRNA TranslationProtein Synthesis RegulationLeishmaniaHuman CellsMouse TestisSucrose GradientUltracentrifugationRibosome FractionationCytoplasmic LysateCycloheximideCell CulturePBS WashCell Counting

Read Article

Cite this Article

Karamysheva, Z. N., Tikhonova, E. B., Grozdanov, P. N., Huffman, J. C., Baca, K. R., Karamyshev, A., Denison, R. B., MacDonald, C. C., Zhang, K., Karamyshev, A. L. Polysome Profiling in Leishmania, Human Cells and Mouse Testis. J. Vis. Exp. (134), e57600, doi:10.3791/57600 (2018).

Download .ris file

Copy

Share Video

.

Copy