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Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro
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Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro
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09:33 min
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June 02, 2018
DOI:
1Program in Developmental Biology,Baylor Tıp Fakültesi, 2Center for Cell and Gene Therapy, Texas Children’s Hospital, and Houston Methodist Hospital,Baylor Tıp Fakültesi, 3Molecular and Cellular Biology Department,Baylor Tıp Fakültesi, 4Stem Cell and Regenerative Medicine Center,Baylor Tıp Fakültesi, 5McNair Medical Institute,Baylor Tıp Fakültesi
Özet
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Here, we present a protocol to generate insulin expressing 3D murine pancreatoids from free-floating e10.5 dissociated pancreatic progenitors and the associated mesenchyme.
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Scavuzzo, M. A., Teaw, J., Yang, D., Borowiak, M. Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro. J. Vis. Exp. (136), e57599, doi:10.3791/57599 (2018).
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