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Genetics
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从土壤、根际和根 Endosphere 提取细菌群落数据的根菌群探析
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JoVE Journal
Genetics
Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
Please note that all translations are automatically generated.
Click here for the English version.
从土壤、根际和根 Endosphere 提取细菌群落数据的根菌群探析
DOI:
10.3791/57561-v
•
09:55 min
•
May 02, 2018
•
Tuesday Simmons
,
Daniel F. Caddell
,
Siwen Deng
,
Devin Coleman-Derr
2
1
Department of Plant and Microbial Biology
,
University of California, Berkeley
,
2
Plant Gene Expression Center
,
USDA ARS
Chapters
00:05
Title
00:46
Collection and Separation of Root Endosphere, Rhizosphere, and Soil Samples
02:47
DNA Extraction
04:11
Amplicon Library Preparation and Submission
08:21
Results: Effective DNA Amplification
09:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们描述了获取土壤、根际和根 endosphere 微生物的扩增子序列数据的协议。该信息可用于研究植物相关微生物群落的组成和多样性, 适合广泛的植物种类使用。
Tags
Root Microbiome
Soil
Rhizosphere
Root Endosphere
16S Ribosomal RNA Gene
Community Profiling
Microbial Ecology
DNA Extraction
Sequencing Library
Soil Core
Rhizosphere Separation
Root Washing
DNA Extraction
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