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Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
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遗传学
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JoVE 杂志 遗传学
Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

DOI:
10.3791/57561-v

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09:55 min

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May 02, 2018

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, , ,
1Department of Plant and Microbial Biology,University of California, Berkeley, 2Plant Gene Expression Center,USDA ARS

Chapters

  • 00:05Title
  • 00:46Collection and Separation of Root Endosphere, Rhizosphere, and Soil Samples
  • 02:47DNA Extraction
  • 04:11Amplicon Library Preparation and Submission
  • 08:21Results: Effective DNA Amplification
  • 09:10Conclusion

Summary

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Here, we describe a protocol to obtain amplicon sequence data of soil, rhizosphere, and root endosphere microbiomes. This information can be used to investigate the composition and diversity of plant-associated microbial communities, and is suitable for the use with a wide range of plant species.

Tags

Root MicrobiomeSoilRhizosphereRoot Endosphere16S Ribosomal RNA GeneCommunity ProfilingMicrobial EcologyDNA ExtractionSequencing LibrarySoil CoreRhizosphere SeparationRoot WashingDNA Extraction

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