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Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
JoVE Journal
Genetica
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JoVE Journal Genetica
Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

24,447 Views

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09:55 min

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May 02, 2018

DOI:

10.3791/57561-v

DOI:
10.3791/57561-v

•

09:55 min
May 02, 2018

•

27 Views
, , ,
1Department of Plant and Microbial Biology,University of California, Berkeley, 2Plant Gene Expression Center,USDA ARS

Summary

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Here, we describe a protocol to obtain amplicon sequence data of soil, rhizosphere, and root endosphere microbiomes. This information can be used to investigate the composition and diversity of plant-associated microbial communities, and is suitable for the use with a wide range of plant species.

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Cite this Article

Simmons, T., Caddell, D. F., Deng, S., Coleman-Derr, D. Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere. J. Vis. Exp. (135), e57561, doi:10.3791/57561 (2018).

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