Rivista
/
Genetica
/
Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
JoVE Journal
Genetica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Genetica
Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

24,361 Views

•

09:55 min

•

May 02, 2018

DOI:

10.3791/57561-v

DOI:
10.3791/57561-v

•

09:55 min
May 02, 2018

•

27 Views
, , ,
1Department of Plant and Microbial Biology,University of California, Berkeley, 2Plant Gene Expression Center,USDA ARS

Summary

Automatically generated

Here, we describe a protocol to obtain amplicon sequence data of soil, rhizosphere, and root endosphere microbiomes. This information can be used to investigate the composition and diversity of plant-associated microbial communities, and is suitable for the use with a wide range of plant species.

Explore More Videos

Root MicrobiomeSoilRhizosphereRoot Endosphere16S Ribosomal RNA GeneCommunity ProfilingMicrobial EcologyDNA ExtractionSequencing LibrarySoil CoreRhizosphere SeparationRoot WashingDNA Extraction

Read Article

Cite this Article

Simmons, T., Caddell, D. F., Deng, S., Coleman-Derr, D. Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere. J. Vis. Exp. (135), e57561, doi:10.3791/57561 (2018).

Download .ris file

Copy

Share Video

.

Copy