Journal
/
유전학
/
Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
JoVE 신문
유전학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 유전학
Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

24,361 Views

•

09:55 min

•

May 02, 2018

DOI:

10.3791/57561-v

DOI:
10.3791/57561-v

•

09:55 min
May 02, 2018

•

27 Views
, , ,
1Department of Plant and Microbial Biology,University of California, Berkeley, 2Plant Gene Expression Center,USDA ARS

Summary

Automatically generated

Here, we describe a protocol to obtain amplicon sequence data of soil, rhizosphere, and root endosphere microbiomes. This information can be used to investigate the composition and diversity of plant-associated microbial communities, and is suitable for the use with a wide range of plant species.

Explore More Videos

Root MicrobiomeSoilRhizosphereRoot Endosphere16S Ribosomal RNA GeneCommunity ProfilingMicrobial EcologyDNA ExtractionSequencing LibrarySoil CoreRhizosphere SeparationRoot WashingDNA Extraction

Read Article

Cite this Article

Simmons, T., Caddell, D. F., Deng, S., Coleman-Derr, D. Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere. J. Vis. Exp. (135), e57561, doi:10.3791/57561 (2018).

Download .ris file

Copy

Share Video

.

Copy