/

Immunology and Infection

/

小鼠和人源性胃上皮单层培养对再生的研究

JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Immunology and Infection

Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration

Please note that all translations are automatically generated. Click here for the English version.

小鼠和人源性胃上皮单层培养对再生的研究

DOI:

10.3791/57435-v

•

11:48 min

•

May 07, 2018

•

Emma Teal, Nina G. Steele, Jayati Chakrabarti, Loryn Holokai, Yana Zavros

¹Department of Pharmacology and Systems Physiology,University of Cincinnati, ²Department of Cell & Developmental Biology,University of Michigan, ³Department of Molecular Genetics, Biochemistry & Microbiology,University of Cincinnati

Chapters

00:04Title
01:04Establishing Human-derived 3D Gastric Fundic Organoids
05:23Transfer of 3D m/hFGOs to 2D Gastric Epithelial Cell Monolayers
07:44Scratch Wound Assay Using 2D Gastric Epithelial Cell Monolayers
09:12Results: Use of Gastric Epithelial Monolayer as a Scratch-wound Assay for Gastric Regeneration
10:53Conclusion

Summary

Automatic Translation

在这里, 我们描述了建立和培养人和老鼠衍生的3维 (3D) 胃 organoids 的协议, 以及将 3D organoids 转移到2维单层的方法。本文还介绍了用胃上皮单层作为再生研究的一种新的擦伤创面检测方法。

Tags

Gastric Epithelial Monolayer Primary Cell Culture Mouse Human Regeneration Wound Healing Helicobacter Pylori Epithelial Immune Cell Co-culture Primary Gastric Cells Organoid Generation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

鼠标应用结扎Peyer氏修补肠袢含量评价M细胞的细菌吸收

神经元的初步培养体系的研究疱疹病毒潜伏期和活化

主要鼠Ⅱ型肺泡上皮细胞的功能及分子生物学研究的流式细胞仪分离

建立的液体覆盖的文化偏光人类呼吸道上皮的CALU-3细胞研究呼吸系统病原宿主细胞的反应<em在体外</em

打印温敏反向模具图案创作的双组分水凝胶的三维细胞培养

上皮损伤制作分析<em>阿米巴</em>感染

幽门螺杆菌感染与胃病理的小鼠模型

原发性鸡初囊-细胞培养模型研究传染性囊内病病毒发病机制

小鼠小肠上皮类器官与先天性淋巴细胞的共培养

用于过敏性接触性皮炎评估的小鼠耳模型

Read Article