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Mouse e humanos derivada de cultura de monocamada epitelial gástrica primária para o estudo da regeneração
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Immunology and Infection
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JoVE Journal Immunology and Infection
Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration

Mouse e humanos derivada de cultura de monocamada epitelial gástrica primária para o estudo da regeneração

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10,262 Views

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11:48 min

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May 07, 2018

DOI:

10.3791/57435-v

DOI:
10.3791/57435-v

•

11:48 min
May 07, 2018

•

12 Views
, , , ,
1Department of Pharmacology and Systems Physiology,University of Cincinnati, 2Department of Cell & Developmental Biology,University of Michigan, 3Department of Molecular Genetics, Biochemistry & Microbiology,University of Cincinnati

Summary

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Aqui descrevemos um protocolo para o estabelecimento e cultivo humano e rato derivada de 3-dimensional organoids gástrica (3D) e o método de transferência de organoids 3D para um monolayer 2-dimensional. O uso da monocamada epitelial gástrica como um romance ensaio de zero-ferida para estudos de regeneração também é descrito.

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Teal, E., Steele, N. G., Chakrabarti, J., Holokai, L., Zavros, Y. Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration. J. Vis. Exp. (135), e57435, doi:10.3791/57435 (2018).

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