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Mouse e umano-derivato cultura dello strato monomolecolare epiteliale gastrica primaria per lo studio della rigenerazione
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Immunology and Infection
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JoVE Journal Immunology and Infection
Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration
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Mouse e umano-derivato cultura dello strato monomolecolare epiteliale gastrica primaria per lo studio della rigenerazione

italiano

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10,262 Views

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11:48 min

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May 07, 2018

DOI:

10.3791/57435-v

DOI:
10.3791/57435-v

•

11:48 min
May 07, 2018

•

12 Views
, , , ,
1Department of Pharmacology and Systems Physiology,University of Cincinnati, 2Department of Cell & Developmental Biology,University of Michigan, 3Department of Molecular Genetics, Biochemistry & Microbiology,University of Cincinnati

Summary

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Qui descriviamo un protocollo per stabilire e coltura umano e del mouse-derivato organoids gastrico 3-dimensionale (3D) e il metodo per il trasferimento di organoids 3D di un monostrato di 2-dimensionale. L'uso del monolayer epiteliale gastrico come un'analisi novella di zero-ferita per studi di rigenerazione è anche descritto.

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Cite this Article

Teal, E., Steele, N. G., Chakrabarti, J., Holokai, L., Zavros, Y. Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration. J. Vis. Exp. (135), e57435, doi:10.3791/57435 (2018).

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