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Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration

Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration

10,262 Views

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11:48 min

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May 07, 2018

DOI:

10.3791/57435-v

DOI:
10.3791/57435-v

•

11:48 min
May 07, 2018

•

12 Views
, , , ,
1Department of Pharmacology and Systems Physiology,University of Cincinnati, 2Department of Cell & Developmental Biology,University of Michigan, 3Department of Molecular Genetics, Biochemistry & Microbiology,University of Cincinnati

Summary

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Here we describe a protocol for establishing and culturing human- and mouse-derived 3-dimensional (3D) gastric organoids, and the method for the transfer of 3D organoids to a 2-dimensional monolayer. The use of the gastric epithelial monolayer as a novel scratch-wound assay for regeneration studies is also described.

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Teal, E., Steele, N. G., Chakrabarti, J., Holokai, L., Zavros, Y. Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration. J. Vis. Exp. (135), e57435, doi:10.3791/57435 (2018).

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