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An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis
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October 04, 2018
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Here we describe the isolation of chicken primary bursal cells from the bursa of Fabricius, the culture and infection of the cells with infectious bursal disease virus, and the quantification of viral replication.
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Dulwich, K. L., Asfor, A. S., Gray, A. G., Nair, V., Broadbent, A. J. An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis. J. Vis. Exp. (140), e58489, doi:10.3791/58489 (2018).
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