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カスパーゼ活性化分子蛍光補完への道をライトアップ
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Biochemistry
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JoVE Journal Biochemistry
Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation
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カスパーゼ活性化分子蛍光補完への道をライトアップ

日本語

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8,906 Views

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08:47 min

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March 05, 2018

DOI:

10.3791/57316-v

DOI:
10.3791/57316-v

•

08:47 min
March 05, 2018

•

4 Views
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1Department of Pediatrics, Division of Hematology-Oncology,Baylor College of Medicine, 2Department of Pediatrics, Division of Hematology-Oncology and Department of Molecular and Cellular Biology,Baylor College of Medicine

Summary

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このプロトコルを記述するカスパーゼ分子蛍光補完 (BiFC);自分の活性化の最初のステップであるイニシエーター カスパーゼの誘起近接を視覚化に使用することができますイメージング ベースのメソッド。

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Cite this Article

Charendoff, C. I., Bouchier-Hayes, L. Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation. J. Vis. Exp. (133), e57316, doi:10.3791/57316 (2018).

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