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Illuminant les voies à l’Activation des caspases utilisant la Fluorescence bimoléculaire complémentation
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Biochemistry
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JoVE Journal Biochemistry
Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation
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Illuminant les voies à l’Activation des caspases utilisant la Fluorescence bimoléculaire complémentation

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8,906 Views

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08:47 min

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March 05, 2018

DOI:

10.3791/57316-v

DOI:
10.3791/57316-v

•

08:47 min
March 05, 2018

•

4 Views
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1Department of Pediatrics, Division of Hematology-Oncology,Baylor College of Medicine, 2Department of Pediatrics, Division of Hematology-Oncology and Department of Molecular and Cellular Biology,Baylor College of Medicine

Summary

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Ce protocole décrit la caspase bimoléculaire complémentation de Fluorescence (BiFC) ; une méthode axée sur l’imagerie qui permet de visualiser la proximité induite par des caspases initiateur, qui est la première étape de leur activation.

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Cite this Article

Charendoff, C. I., Bouchier-Hayes, L. Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation. J. Vis. Exp. (133), e57316, doi:10.3791/57316 (2018).

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