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Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation
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JoVE Journal Biochimica
Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

8,903 Views

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08:47 min

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March 05, 2018

DOI:

10.3791/57316-v

DOI:
10.3791/57316-v

•

08:47 min
March 05, 2018

•

4 Views
,
1Department of Pediatrics, Division of Hematology-Oncology,Baylor College of Medicine, 2Department of Pediatrics, Division of Hematology-Oncology and Department of Molecular and Cellular Biology,Baylor College of Medicine

Summary

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This protocol describes caspase Bimolecular Fluorescence Complementation (BiFC); an imaging-based method that can be used to visualize induced proximity of initiator caspases, which is the first step in their activation.

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Cite this Article

Charendoff, C. I., Bouchier-Hayes, L. Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation. J. Vis. Exp. (133), e57316, doi:10.3791/57316 (2018).

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