Journal
/
Biochemie
/
Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation
JoVE Journal
Biochemie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Biochemie
Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

8,906 Views

•

08:47 min

•

March 05, 2018

DOI:

10.3791/57316-v

DOI:
10.3791/57316-v

•

08:47 min
March 05, 2018

•

4 Views
,
1Department of Pediatrics, Division of Hematology-Oncology,Baylor College of Medicine, 2Department of Pediatrics, Division of Hematology-Oncology and Department of Molecular and Cellular Biology,Baylor College of Medicine

Summary

Automatically generated

This protocol describes caspase Bimolecular Fluorescence Complementation (BiFC); an imaging-based method that can be used to visualize induced proximity of initiator caspases, which is the first step in their activation.

Explore More Videos

Caspase ActivationBimolecular Fluorescence ComplementationInduced ProximityApoptotic Cell DeathInitiator CaspasesTransfectionReporter PlasmidBiFC PlasmidDNA Lipid ComplexMicroscopy

Read Article

Cite this Article

Charendoff, C. I., Bouchier-Hayes, L. Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation. J. Vis. Exp. (133), e57316, doi:10.3791/57316 (2018).

Download .ris file

Copy

Share Video

.

Copy