Journal
/
Bioquímica
/
Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

DOI:
10.3791/57316-v

•

08:47 min

•

March 05, 2018

•

,
1Department of Pediatrics, Division of Hematology-Oncology,Baylor College of Medicine, 2Department of Pediatrics, Division of Hematology-Oncology and Department of Molecular and Cellular Biology,Baylor College of Medicine

Capítulos

  • 00:04Título
  • 00:36Transfection of Cells to Introduce the Caspase Bimolecular Fluorescence Complementation (BiFC) Components
  • 02:42Three-dimensional Imaging of Caspase BiFC
  • 05:00Time-lapse Imaging of Caspase BiFC
  • 06:19Results: Visualization of Caspase Activation Pathways Using Caspase Bimolecular Fluorescence Complementation
  • 07:37Conclusion

Summary

Tadução automática

Tadução automática

This protocol describes caspase Bimolecular Fluorescence Complementation (BiFC); an imaging-based method that can be used to visualize induced proximity of initiator caspases, which is the first step in their activation.

Tags

Caspase ActivationBimolecular Fluorescence ComplementationInduced ProximityApoptotic Cell DeathInitiator CaspasesTransfectionReporter PlasmidBiFC PlasmidDNA Lipid ComplexMicroscopy

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging

Complementation of Splicing Activity by a Galectin-3 – U1 snRNP Complex on Beads

Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry

A Knowledge Graph Approach to Elucidate the Role of Organellar Pathways in Disease via Biomedical Reports

Read Article