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Preparación de glándula salival submaxilar murino para la microscopia Intravital vertical
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy
DOI:

08:13 min

May 07, 2018

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Chapters

  • 00:05Title
  • 00:39Removal of the Fur and Fixation of the Mouse on the Stage
  • 02:46Surgical Exposure of the Salivary Gland Using a Stereomicroscope
  • 04:09Fixing of the Gland, Heating Ring, and the Temperature Probe
  • 06:10Results: Use of Immunofluorescence Imaging to Study the Submandibular Gland
  • 07:19Conclusion

Summary

Automatic Translation

Se describe un protocolo para exponer quirúrgicamente y estabilizar la glándula salival submaxilar murina intravital imagen usando microscopia intravital vertical. Este protocolo es fácilmente adaptable a otras glándulas exocrinas de la cabeza y región del cuello de los ratones y otros roedores pequeños.

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