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Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy
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JoVE 신문 면역학 및 감염병학
Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy

Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy

7,893 Views

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08:13 min

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May 07, 2018

DOI:

10.3791/57283-v

DOI:
10.3791/57283-v

•

08:13 min
May 07, 2018

•

23 Views
, , ,
1Theodor-Kocher Institute,University of Bern, 2Center for Infectious Diseases, Integrative Virology,University Clinic of Heidelberg

Summary

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We describe a protocol to surgically expose and stabilize the murine submandibular salivary gland for intravital imaging using upright intravital microscopy. This protocol is easily adaptable to other exocrine glands of the head and neck region of mice and other small rodents.

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Ficht, X., Thelen, F., Stolp, B., Stein, J. V. Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy. J. Vis. Exp. (135), e57283, doi:10.3791/57283 (2018).

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