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Developmental Biology
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Pax7 和层粘连蛋白抗体免疫荧光法鉴定骨骼肌卫星细胞
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Developmental Biology
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JoVE Journal
Developmental Biology
Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies
Please note that all translations are automatically generated.
Click here for the English version.
Pax7 和层粘连蛋白抗体免疫荧光法鉴定骨骼肌卫星细胞
DOI:
10.3791/57212-v
•
07:18 min
•
April 19, 2018
•
Xuesong Feng*
1
,
Faiza Naz*
2
,
Aster H. Juan
,
Stefania Dell’Orso
,
Vittorio Sartorelli
1
Laboratory of Muscle Stem Cells and Gene Regulation
,
National Institutes of Health
,
2
Office of Science and Technology, National Institute of Arthritis, Musculoskeletal and Skin Diseases (NIAMS)
,
National Institutes of Health
Chapters
00:05
Title
00:41
Cryo-embedding of the Tibialis Anterior Muscle
01:46
Cryostat Sectioning
02:33
Immunofluorescence and Staining
05:27
Results: Visualization of Satellite Cells in Adult Resting Muscle Sections is Improved Using Immunofluorescence-based Staining
06:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
卫星细胞的精确识别对于研究其在各种生理和病理条件下的功能至关重要。本文提出了一种基于免疫荧光染色的成人骨骼肌切片卫星细胞识别的协议。
Tags
Skeletal Muscle
Satellite Cells
Immunofluorescence
Pax7
Laminin
Cryosectioning
Cryostat
Antigen Retrieval
Immunostaining
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