Journal
/
Developmental Biology
/
Pax7 和层粘连蛋白抗体免疫荧光法鉴定骨骼肌卫星细胞
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies
Please note that all translations are automatically generated. Click here for the English version.

Pax7 和层粘连蛋白抗体免疫荧光法鉴定骨骼肌卫星细胞

DOI:
10.3791/57212-v

•

07:18 min

•

April 19, 2018

•

, , , ,
1Laboratory of Muscle Stem Cells and Gene Regulation,National Institutes of Health, 2Office of Science and Technology, National Institute of Arthritis, Musculoskeletal and Skin Diseases (NIAMS),National Institutes of Health

Chapters

  • 00:05Title
  • 00:41Cryo-embedding of the Tibialis Anterior Muscle
  • 01:46Cryostat Sectioning
  • 02:33Immunofluorescence and Staining
  • 05:27Results: Visualization of Satellite Cells in Adult Resting Muscle Sections is Improved Using Immunofluorescence-based Staining
  • 06:40Conclusion

Summary

Automatic Translation

Automatic Translation

卫星细胞的精确识别对于研究其在各种生理和病理条件下的功能至关重要。本文提出了一种基于免疫荧光染色的成人骨骼肌切片卫星细胞识别的协议。

Tags

Skeletal MuscleSatellite CellsImmunofluorescencePax7LamininCryosectioningCryostatAntigen RetrievalImmunostaining

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

从人类骨骼肌成肌原细胞和成纤维细胞的分离和定量免疫细胞化学表征

隔离和卫星细胞的表征鼠头Branchiomeric肌肉

斑马鱼幼虫骨骼肌诚信与伊文思蓝分析

通过注射心脏毒素急性骨骼肌再生的诱导

制备及文化活动从成年和老年人类骨骼肌成肌前体细胞/成肌细胞主要的

使用层粘连蛋白521矩阵整合人诱导多能干细胞的自由衍生

慢性刺激在收缩活动性大鼠骨骼肌表型适应性研究中的应用

从骨骼肌活检程序分离、培养、表征和分化人类肌肉祖细胞

在免疫缺陷小鼠中表演人类骨骼肌异种移植物

小鼠骨骼肌肉外泄原发性肌基器的分离和分化

Read Article