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Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies

Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies

20,040 Views

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07:18 min

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April 19, 2018

DOI:

10.3791/57212-v

DOI:
10.3791/57212-v

•

07:18 min
April 19, 2018

•

31 Views
, , , ,
1Laboratory of Muscle Stem Cells and Gene Regulation,National Institutes of Health, 2Office of Science and Technology, National Institute of Arthritis, Musculoskeletal and Skin Diseases (NIAMS),National Institutes of Health

Summary

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The precise identification of satellite cells is essential for studying their functions under various physiological and pathological conditions. This article presents a protocol to identify satellite cells on adult skeletal muscle sections by immunofluorescence-based staining.

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Skeletal MuscleSatellite CellsImmunofluorescencePax7LamininCryosectioningCryostatAntigen RetrievalImmunostaining

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Cite this Article

Feng, X., Naz, F., Juan, A. H., Dell'Orso, S., Sartorelli, V. Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies. J. Vis. Exp. (134), e57212, doi:10.3791/57212 (2018).

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