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Pax7 とラミニン抗体抗体法による骨格筋衛星細胞の同定
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Developmental Biology
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JoVE Journal Developmental Biology
Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies
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Pax7 とラミニン抗体抗体法による骨格筋衛星細胞の同定

日本語

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20,044 Views

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07:18 min

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April 19, 2018

DOI:

10.3791/57212-v

DOI:
10.3791/57212-v

•

07:18 min
April 19, 2018

•

31 Views
, , , ,
1Laboratory of Muscle Stem Cells and Gene Regulation,National Institutes of Health, 2Office of Science and Technology, National Institute of Arthritis, Musculoskeletal and Skin Diseases (NIAMS),National Institutes of Health

Summary

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衛星細胞の正確な同定は様々 な生理学的、病理学的条件の下でその機能を研究するために不可欠です。この記事では、蛍光抗体法による染色による大人の骨格筋のセクションの衛星細胞を識別するためにプロトコルを示します。

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Cite this Article

Feng, X., Naz, F., Juan, A. H., Dell'Orso, S., Sartorelli, V. Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies. J. Vis. Exp. (134), e57212, doi:10.3791/57212 (2018).

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