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Identificatie van de skeletspieren satelliet cellen met immunofluorescentie met Pax7 en Laminin antilichamen
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Developmental Biology
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JoVE Journal Developmental Biology
Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies
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Identificatie van de skeletspieren satelliet cellen met immunofluorescentie met Pax7 en Laminin antilichamen

Nederlands

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20,044 Views

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07:18 min

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April 19, 2018

DOI:

10.3791/57212-v

DOI:
10.3791/57212-v

•

07:18 min
April 19, 2018

•

31 Views
, , , ,
1Laboratory of Muscle Stem Cells and Gene Regulation,National Institutes of Health, 2Office of Science and Technology, National Institute of Arthritis, Musculoskeletal and Skin Diseases (NIAMS),National Institutes of Health

Summary

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De nauwkeurige identificatie van satelliet cellen is essentieel voor de studie van hun functies onder verschillende fysiologische en pathologische omstandigheden. Dit artikel presenteert een protocol ter identificatie van de satelliet cellen op volwassen skeletspieren secties door immunofluorescentie gebaseerde kleuring.

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Feng, X., Naz, F., Juan, A. H., Dell'Orso, S., Sartorelli, V. Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies. J. Vis. Exp. (134), e57212, doi:10.3791/57212 (2018).

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