Journal
/
Genetics
/
Un método de Optogenetic para controlar y analizar patrones de expresión génica en las interacciones célula a célula
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
Please note that all translations are automatically generated. Click here for the English version.

Un método de Optogenetic para controlar y analizar patrones de expresión génica en las interacciones célula a célula

español

Automatically Generated

7,511 Views

•

07:59 min

•

March 22, 2018

DOI:

10.3791/57149-v

DOI:
10.3791/57149-v

•

07:59 min
March 22, 2018

•

1 Views
,
1Institute for Frontier Life and Medical Sciences,Kyoto University, 2Japan Science and Technology Agency,PRESTO, 3Institute for Integrated Cell-Material Sciences,Kyoto University, 4Graduate School of Medicine,Kyoto University, 5Graduate School of Biostudies,Kyoto University

Summary

Automatically generated

Aquí, presentamos un protocolo para analizar la transferencia de célula a célula de información oscilatoria por control optogenetic y control de la expresión génica en vivo. Este enfoque proporciona una plataforma única para probar una significación funcional de los programas de dinámica gene expresión en sistemas multicelulares.

Explore More Videos

OptogeneticsCell-to-cell InteractionsNotch Signaling PathwayGene Expression DynamicsTol2-based Optogenetic ModulesC2C12 CellsFluorescent Protein SelectionLight-induced ConditionsProgrammable Light Illumination

Read Article

Cite this Article

Isomura, A., Kageyama, R. An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions. J. Vis. Exp. (133), e57149, doi:10.3791/57149 (2018).

Download .ris file

Copy

Share Video

.

Copy