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Een Optogenetic methode te controleren en analyseren van gen expressiepatronen in cel-naar-cel interacties
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Genetics
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JoVE Journal Genetics
An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
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Een Optogenetic methode te controleren en analyseren van gen expressiepatronen in cel-naar-cel interacties

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7,511 Views

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07:59 min

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March 22, 2018

DOI:

10.3791/57149-v

DOI:
10.3791/57149-v

•

07:59 min
March 22, 2018

•

1 Views
,
1Institute for Frontier Life and Medical Sciences,Kyoto University, 2Japan Science and Technology Agency,PRESTO, 3Institute for Integrated Cell-Material Sciences,Kyoto University, 4Graduate School of Medicine,Kyoto University, 5Graduate School of Biostudies,Kyoto University

Summary

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Hier presenteren we een protocol te analyseren van cel naar cel overdracht van oscillerende informatie door optogenetic control en live monitoring van genexpressie. Deze aanpak biedt een uniek platform om te testen een functionele betekenis van dynamische gen expressie programma's in meercellige systemen.

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Cite this Article

Isomura, A., Kageyama, R. An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions. J. Vis. Exp. (133), e57149, doi:10.3791/57149 (2018).

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