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细胞间相互作用中基因表达模式的控制与分析的 Optogenetic 方法
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Genetics
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JoVE Journal Genetics
An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
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细胞间相互作用中基因表达模式的控制与分析的 Optogenetic 方法

DOI:
10.3791/57149-v

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07:59 min

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March 22, 2018

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,
1Institute for Frontier Life and Medical Sciences,Kyoto University, 2Japan Science and Technology Agency,PRESTO, 3Institute for Integrated Cell-Material Sciences,Kyoto University, 4Graduate School of Medicine,Kyoto University, 5Graduate School of Biostudies,Kyoto University

Chapters

  • 00:04Title
  • 00:44Generation of Stable Cell Lines by the Tol2 System
  • 02:07Evaluation of Photo-sensitivity of Engineered Cells at the Population Level
  • 03:31Dynamic Sender-receiver Assay at the Population Level and at the Single-cell Level
  • 06:47Results: Analysis of Gene Expression Patterns in Cell-to-cell Interactions
  • 07:35Conclusion

Summary

Automatic Translation

Automatic Translation

本文提出了一种通过 optogenetic 控制和实时监测基因表达来分析振荡信息的细胞间传递的协议。这种方法为测试多细胞系统动态基因表达程序的功能意义提供了一个独特的平台。

Tags

OptogeneticsCell-to-cell InteractionsNotch Signaling PathwayGene Expression DynamicsTol2-based Optogenetic ModulesC2C12 CellsFluorescent Protein SelectionLight-induced ConditionsProgrammable Light Illumination

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