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JoVE Journal Genetics
An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
 

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

Article DOI: 10.3791/57149-v • 07:59 min • March 22nd, 2018
March 22nd, 2018 •
1,2, 1,3,4,5
1Institute for Frontier Life and Medical Sciences, Kyoto University, 2Japan Science and Technology Agency, PRESTO, 3Institute for Integrated Cell-Material Sciences, Kyoto University, 4Graduate School of Medicine, Kyoto University, 5Graduate School of Biostudies, Kyoto University

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Here, we present a protocol to analyze cell-to-cell transfer of oscillatory information by optogenetic control and live monitoring of gene expression. This approach provides a unique platform to test a functional significance of dynamic gene expression programs in multicellular systems.

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Optogenetic Method Control Gene Expression Patterns Cell-to-cell Interactions Oscillatory Information Notch Signaling Pathway Gene Expression Dynamics Akihiro Isomura Tol2-based Optogenetic Modules Transfection Cell Counter Drug Selection Vector Transposase Expression Vector Lipofection Reagent Culture Medium Hygromycin
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