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小鼠膈运动神经元逆行神经解剖学追踪的研究
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Neuroscience
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JoVE Journal Neuroscience
Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice
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小鼠膈运动神经元逆行神经解剖学追踪的研究

DOI:
10.3791/56758-v

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09:05 min

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February 22, 2018

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, , , ,
1URVI, NARILIS,Université de Namur, 2URPhyM, NARILIS,Université de Namur

Chapters

  • 00:05Title
  • 01:23Preparations
  • 02:22Intrapleural Injections Using Transdiaphragmatic Approach
  • 04:48Intrapleural Injection using Transthoracic Approach and Post-operative Care
  • 06:38Results: Labeling Neurons with CTB after a C4 Spinal Contusion
  • 08:01Conclusion

Summary

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Automatic Translation

本文描述了荧光共轭霍乱毒素亚单位β胸腔内分娩后小鼠膈运动神经元识别的一种协议。比较两种方法注射胸腔: 膈与经胸的方法。

Tags

Retrograde Neuroanatomical TracingPhrenic Motor NeuronsMiceIntrapleural DeliveryFluorophore-conjugated Cholera Toxin Subunit BetaTransdiaphragmatic ApproachTransthoracic ApproachNeuromuscular JunctionsPleural SpaceSterile SurgeryAnesthesiaSkin PreparationCTB SolutionMicrosyringeLaparotomy

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