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Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice
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February 22, 2018
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Here, we describe a protocol for identifying phrenic motor neurons in mice after intrapleural delivery of fluorophore conjugated cholera toxin subunit beta. Two techniques are compared to inject the pleural cavity: transdiaphragmatic versus transthoracic approaches.
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Vandeweerd, J., Hontoir, F., De Knoop, A., De Swert, K., Nicaise, C. Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice. J. Vis. Exp. (132), e56758, doi:10.3791/56758 (2018).
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