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Rastreamento neuroanatômica retrógrado do frênico neurônios em ratos
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Neuroscience
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JoVE Journal Neuroscience
Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice

Rastreamento neuroanatômica retrógrado do frênico neurônios em ratos

português

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12,309 Views

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09:05 min

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February 22, 2018

DOI:

10.3791/56758-v

DOI:
10.3791/56758-v

•

09:05 min
February 22, 2018

•

11 Views
, , , ,
1URVI, NARILIS,Université de Namur, 2URPhyM, NARILIS,Université de Namur

Summary

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Aqui, descrevemos um protocolo para identificar frênico neurônios em ratos após entrega intrapleural de fluoróforo conjugado beta de subunidade de toxina de cólera. Duas técnicas são comparadas para injetar a cavidade pleural: transdiaphragmatic versus transtorácicos abordagens.

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Vandeweerd, J., Hontoir, F., De Knoop, A., De Swert, K., Nicaise, C. Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice. J. Vis. Exp. (132), e56758, doi:10.3791/56758 (2018).

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