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Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice
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JoVE Journal Neurowissenschaften
Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice

Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice

12,309 Views

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09:05 min

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February 22, 2018

DOI:

10.3791/56758-v

DOI:
10.3791/56758-v

•

09:05 min
February 22, 2018

•

11 Views
, , , ,
1URVI, NARILIS,Université de Namur, 2URPhyM, NARILIS,Université de Namur

Summary

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Here, we describe a protocol for identifying phrenic motor neurons in mice after intrapleural delivery of fluorophore conjugated cholera toxin subunit beta. Two techniques are compared to inject the pleural cavity: transdiaphragmatic versus transthoracic approaches.

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Retrograde Neuroanatomical TracingPhrenic Motor NeuronsMiceIntrapleural DeliveryFluorophore-conjugated Cholera Toxin Subunit BetaTransdiaphragmatic ApproachTransthoracic ApproachNeuromuscular JunctionsPleural SpaceSterile SurgeryAnesthesiaSkin PreparationCTB SolutionMicrosyringeLaparotomy

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Vandeweerd, J., Hontoir, F., De Knoop, A., De Swert, K., Nicaise, C. Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice. J. Vis. Exp. (132), e56758, doi:10.3791/56758 (2018).

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