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Neuroscience
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产后大鼠听觉脑干反应及外毛细胞全细胞贴片钳记录
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Neuroscience
Auditory Brainstem Response and Outer Hair Cell Whole-cell Patch Clamp Recording in Postnatal Rats
Please note that all translations are automatically generated.
Click here for the English version.
产后大鼠听觉脑干反应及外毛细胞全细胞贴片钳记录
DOI:
10.3791/56678-v
•
09:23 min
•
May 24, 2018
•
Aoshuang Chang
2
,
Cuixian Li
,
Jianfeng Huang
3
,
Wenlu Pan
,
Yinghong Tian
,
Jie Tang
1
Department of Physiology, School of Basic Medical Sciences
,
Southern Medical University
,
2
School of Basic Medical Sciences
,
Guizhou Medical University
,
3
Department of Laboratory Medicines, Guangzhou General Hospital of Guangzhou Military Region
,
Southern Medical University
,
4
Experiment Teaching Center, School of Basic Medical Sciences
,
Southern Medical University
Chapters
00:04
Title
00:56
Auditory Brainstem Response Recording
02:12
The Organ of Corti Dissection
03:22
Immunofluorescence Staining
05:21
Patch Clamp Recording of Isolated OHCs
07:33
Results: Whole-Cell Patch Clamp Recording of Isolated Outer Hair Cells
08:19
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述的方法, 记录的听觉脑干反应的产后鼠幼崽。为研究外发细胞的功能发育情况, 本文分步介绍了全细胞膜片钳在分离外毛细胞中的实验过程。
Tags
Auditory Brainstem Response
Outer Hair Cell
Whole-cell Patch Clamp
Postnatal Rats
Cochlear Hair Cells
Auditory Function
Electrophysiology
Inner Ear
Cochlea
Organ Of Corti
Leibovitz’s L-15 Medium
Dissection
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