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产后大鼠听觉脑干反应及外毛细胞全细胞贴片钳记录
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Auditory Brainstem Response and Outer Hair Cell Whole-cell Patch Clamp Recording in Postnatal Rats
DOI:

09:23 min

May 24, 2018

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Chapters

  • 00:04Title
  • 00:56Auditory Brainstem Response Recording
  • 02:12The Organ of Corti Dissection
  • 03:22Immunofluorescence Staining
  • 05:21Patch Clamp Recording of Isolated OHCs
  • 07:33Results: Whole-Cell Patch Clamp Recording of Isolated Outer Hair Cells
  • 08:19Conclusion

Summary

Automatic Translation

本协议描述的方法, 记录的听觉脑干反应的产后鼠幼崽。为研究外发细胞的功能发育情况, 本文分步介绍了全细胞膜片钳在分离外毛细胞中的实验过程。

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