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Auditivo de tronco encefálico e exterior cabelo célula célula inteira Patch Clamp gravação em ratos pós-natal
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09:23 min
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May 24, 2018
DOI:
1Department of Physiology, School of Basic Medical Sciences,Southern Medical University, 2School of Basic Medical Sciences,Guizhou Medical University, 3Department of Laboratory Medicines, Guangzhou General Hospital of Guangzhou Military Region,Southern Medical University, 4Experiment Teaching Center, School of Basic Medical Sciences,Southern Medical University
Summary
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Este protocolo descreve métodos para gravar a resposta auditiva do tronco encefálico de filhotes pós-natal. Para examinar o desenvolvimento funcional de células exteriores do cabelo, o procedimento experimental da braçadeira do remendo de células inteiras gravação em células isoladas de cabelo exteriores é descrito passo a passo.
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Chang, A., Li, C., Huang, J., Pan, W., Tian, Y., Tang, J. Auditory Brainstem Response and Outer Hair Cell Whole-cell Patch Clamp Recording in Postnatal Rats. J. Vis. Exp. (135), e56678, doi:10.3791/56678 (2018).
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