/
/
Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins
A subscription to JoVE is required to view this content. Sign in or start your free trial.
16,645 Views
•
08:39 min
•
May 22, 2017
DOI:
Summary
Automatically generated
This article describes how the ion selectivity of channelrhodopsin is determined with electrophysiological whole-cell patch-clamp recordings using HEK293 cells. Here, the experimental procedure for investigating chloride selectivity of an anion-selective channelrhodopsin is demonstrated. However, the procedure is transferable to other channelrhodopsins of distinct selectivity.
Read Article
Cite this Article
Grimm, C., Vierock, J., Hegemann, P., Wietek, J. Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins. J. Vis. Exp. (123), e55497, doi:10.3791/55497 (2017).
Copy