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JoVE Journal Neuroscience
Auditory Brainstem Response and Outer Hair Cell Whole-cell Patch Clamp Recording in Postnatal Rats
 

Auditory Brainstem Response and Outer Hair Cell Whole-cell Patch Clamp Recording in Postnatal Rats

Article DOI: 10.3791/56678-v • 09:23 min • May 24th, 2018
May 24th, 2018 •
1,2, 1, 1,3, 1, 4, 1
1Department of Physiology, School of Basic Medical Sciences, Southern Medical University, 2School of Basic Medical Sciences, Guizhou Medical University, 3Department of Laboratory Medicines, Guangzhou General Hospital of Guangzhou Military Region, Southern Medical University, 4Experiment Teaching Center, School of Basic Medical Sciences, Southern Medical University

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Summary
Automatic Translation

This protocol describes methods to record the auditory brainstem response from postnatal rat pups. To examine the functional development of outer hair cells, the experimental procedure of whole-cell patch clamp recording in isolated outer hair cells is described step-by-step.

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Auditory Brainstem Response Outer Hair Cell Whole-cell Patch Clamp Recording Postnatal Rats Electrophysiological Assay Morphological Changes Functional Changes Cochlear Hair Cells Postnatal Development Audical Assistant Department Field Hair Cell Functions Hearing Onset Individual Outer Hair Cells Postnatal Rat Pups Wenlu Pan Shasha Guo Nana Xu Anesthetized Animal Polyethylene Foam Mold Anti-vibration Table Sound-attenuating Room Body Temperature Heating Pad Head Area Cleaning 70% Ethanol Incision Ventral-laterally Reference Electrode Ground Electrode Subdermal Recording Electrode Skull Vertex Function Generator Tone Bursts Frequencies And Intensities
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