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Visualización de axón talamocorticales ramificación y sinapsis formación en Organotypic Cocultures
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Neuroscience
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JoVE Journal Neuroscience
Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures
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Visualización de axón talamocorticales ramificación y sinapsis formación en Organotypic Cocultures

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6,354 Views

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06:16 min

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March 28, 2018

DOI:

10.3791/56553-v

DOI:
10.3791/56553-v

•

06:16 min
March 28, 2018

•

1 Views
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1Department of Medical Neuroscience, Graduate School of Medical Sciences,Kanazawa University, 2Neuroscience Laboratories, Graduate School of Frontier Biosciences,Osaka University

Summary

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Este protocolo describe un método para la proyección de imagen simultánea de talamocorticales axon ramas y sinapsis formación en organotypic cocultures del tálamo y corteza cerebral. Axones individuales talamocorticales y sus terminales presinápticos se visualizan mediante una técnica de electroporación unicelular con DsRed y synaptophysin tagged GFP.

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Cite this Article

Matsumoto, N., Yamamoto, N. Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures. J. Vis. Exp. (133), e56553, doi:10.3791/56553 (2018).

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