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Visualisation de Thalamocortical Axon Branching et Synapse Formation chez organotypique Cocultures
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Neuroscience
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JoVE Journal Neuroscience
Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures
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Visualisation de Thalamocortical Axon Branching et Synapse Formation chez organotypique Cocultures

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6,353 Views

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06:16 min

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March 28, 2018

DOI:

10.3791/56553-v

DOI:
10.3791/56553-v

•

06:16 min
March 28, 2018

•

1 Views
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1Department of Medical Neuroscience, Graduate School of Medical Sciences,Kanazawa University, 2Neuroscience Laboratories, Graduate School of Frontier Biosciences,Osaka University

Summary

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Ce protocole décrit une méthode pour l’imagerie simultanée de thalamocortical axon branching et synapse formation dans cocultures organotypique du thalamus et le cortex cérébral. Les axones thalamocortical individuels et leurs terminaisons présynaptiques sont visualisés par une technique d’électroporation monocellulaires avec DsRed et synaptophysine GFP-étiquetée.

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Cite this Article

Matsumoto, N., Yamamoto, N. Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures. J. Vis. Exp. (133), e56553, doi:10.3791/56553 (2018).

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