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Visualisatie van Thalamocortical Axon vertakking en de Synapse formatie in Organotypic Cocultures
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Neuroscience
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JoVE Journal Neuroscience
Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures
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Visualisatie van Thalamocortical Axon vertakking en de Synapse formatie in Organotypic Cocultures

Nederlands

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6,354 Views

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06:16 min

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March 28, 2018

DOI:

10.3791/56553-v

DOI:
10.3791/56553-v

•

06:16 min
March 28, 2018

•

1 Views
,
1Department of Medical Neuroscience, Graduate School of Medical Sciences,Kanazawa University, 2Neuroscience Laboratories, Graduate School of Frontier Biosciences,Osaka University

Summary

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Dit protocol beschrijft een methode voor gelijktijdige beeldvorming van thalamocortical axon vertakking en de synapse formatie in organotypic cocultures van de thalamus en cortex cerebri. Individuele thalamocortical axonen en hun presynaptische terminals worden gevisualiseerd door een eencellige electroporation techniek met DsRed en synaptophysin van GFP-gelabeld.

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Cite this Article

Matsumoto, N., Yamamoto, N. Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures. J. Vis. Exp. (133), e56553, doi:10.3791/56553 (2018).

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