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Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures
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神经科学
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JoVE 杂志 神经科学
Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures

Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures

6,354 Views

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06:16 min

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March 28, 2018

DOI:

10.3791/56553-v

DOI:
10.3791/56553-v

•

06:16 min
March 28, 2018

•

1 Views
,
1Department of Medical Neuroscience, Graduate School of Medical Sciences,Kanazawa University, 2Neuroscience Laboratories, Graduate School of Frontier Biosciences,Osaka University

Summary

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This protocol describes a method for simultaneous imaging of thalamocortical axon branching and synapse formation in organotypic cocultures of the thalamus and cerebral cortex. Individual thalamocortical axons and their presynaptic terminals are visualized by a single cell electroporation technique with DsRed and GFP-tagged synaptophysin.

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Cite this Article

Matsumoto, N., Yamamoto, N. Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures. J. Vis. Exp. (133), e56553, doi:10.3791/56553 (2018).

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