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Visualizzazione di Thalamocortical Axon ramificazione e Synapse formazione in Cocultures organotipiche
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Neuroscience
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JoVE Journal Neuroscience
Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures
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Visualizzazione di Thalamocortical Axon ramificazione e Synapse formazione in Cocultures organotipiche

italiano

Automatically Generated

6,353 Views

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06:16 min

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March 28, 2018

DOI:

10.3791/56553-v

DOI:
10.3791/56553-v

•

06:16 min
March 28, 2018

•

1 Views
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1Department of Medical Neuroscience, Graduate School of Medical Sciences,Kanazawa University, 2Neuroscience Laboratories, Graduate School of Frontier Biosciences,Osaka University

Summary

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Questo protocollo descrive un metodo per l'acquisizione simultanea delle thalamocortical assone ramificazione e sinapsi formazione in organotipiche cocultures del talamo e corteccia cerebrale. Thalamocortical singoli assoni e loro terminali presinaptici sono visualizzati mediante una tecnica di elettroporazione di singola cellula con DsRed e lo synaptophysin GFP-etichettato.

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Cite this Article

Matsumoto, N., Yamamoto, N. Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures. J. Vis. Exp. (133), e56553, doi:10.3791/56553 (2018).

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