Journal
/
Neuroscience
/
Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

English

Automatically Generated

9,607 Views

•

10:39 min

•

February 19, 2018

DOI:

10.3791/56530-v

DOI:
10.3791/56530-v

•

10:39 min
February 19, 2018

•

3 Views
, , , , ,
1Department of Molecular and Cellular Physiology,Stanford University, 2Department of Mechanical Engineering,Stanford University, 3Department of Bioengineering,Stanford University, 4Group of Neurophotonics and Mechanical Systems Biology,The Institute of Photonic Sciences (ICFO)

Summary

Automatically generated

New tools for mechanobiology research are needed to understand how mechanical stress activates biochemical pathways and elicits biological responses. Here, we showcase a new method for selective mechanical stimulation of immobilized animals with a microfluidic trap allowing high-resolution imaging of cellular responses.

Explore More Videos

MicrofluidicsMechanical StimulationHigh-resolution ImagingC. ElegansMechanobiologySensory BiologyPressure ActuatorsGCaMPRFPFluorescence MicroscopyM9 BufferPeristaltic PumpPDMS

Read Article

Cite this Article

Fehlauer, H., Nekimken, A. L., Kim, A. A., Pruitt, B. L., Goodman, M. B., Krieg, M. Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans. J. Vis. Exp. (132), e56530, doi:10.3791/56530 (2018).

Download .ris file

Copy

Share Video

.

Copy