Journal
/
Neurosciences
/
Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans
Journal JoVE
Neurosciences
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Neurosciences
Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

DOI:
10.3791/56530-v

•

10:39 min

•

February 19, 2018

•

, , , , ,
1Department of Molecular and Cellular Physiology,Stanford University, 2Department of Mechanical Engineering,Stanford University, 3Department of Bioengineering,Stanford University, 4Group of Neurophotonics and Mechanical Systems Biology,The Institute of Photonic Sciences (ICFO)

Chapitres

  • 00:05Titre
  • 00:52Preparation of the Microscope
  • 02:02Preparing the Microfluidic Chip
  • 03:45Loading the Test Subject
  • 06:02Stimulation and Removal of the Test Subject
  • 08:13Results: Touch Receptor Neuron (TRN) Stimuli
  • 09:42Conclusion

Summary

Traduction automatique

Traduction automatique

New tools for mechanobiology research are needed to understand how mechanical stress activates biochemical pathways and elicits biological responses. Here, we showcase a new method for selective mechanical stimulation of immobilized animals with a microfluidic trap allowing high-resolution imaging of cellular responses.

Tags

Keywords: MicrofluidicsMechanical StimulationHigh-resolution ImagingC. ElegansMechanobiologySensory BiologyPressure ActuatorsGCaMPRFPFluorescence MicroscopyM9 BufferPeristaltic PumpPDMS

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/56530?article_type=v

Assaying β-amyloid Toxicity using a Transgenic C. elegans Model
check_url/fr/56530?article_type=v

Combining Transcranial Magnetic Stimulation and fMRI to Examine the Default Mode Network
check_url/fr/56530?article_type=v

TMS: Using the Theta-Burst Protocol to Explore Mechanism of Plasticity in Individuals with Fragile X Syndrome and Autism
check_url/fr/56530?article_type=v

State-Dependency Effects on TMS: A Look at Motive Phosphene Behavior
check_url/fr/56530?article_type=v

The NeuroStar TMS Device: Conducting the FDA Approved Protocol for Treatment of Depression
check_url/fr/56530?article_type=v

Enabling High Grayscale Resolution Displays and Accurate Response Time Measurements on Conventional Computers
check_url/fr/56530?article_type=v

High-resolution Functional Magnetic Resonance Imaging Methods for Human Midbrain
check_url/fr/56530?article_type=v

High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium
check_url/fr/56530?article_type=v

Automated Quantification of Synaptic Fluorescence in C. elegans
check_url/fr/56530?article_type=v

In vivo Neuronal Calcium Imaging in C. elegans

Read Article