Dergi
/
Nörobilim
/
Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans
JoVE Journal
Nörobilim
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

9,607 Views

•

10:39 min

•

February 19, 2018

DOI:

10.3791/56530-v

DOI:
10.3791/56530-v

•

10:39 min
February 19, 2018

•

3 Views
, , , , ,
1Department of Molecular and Cellular Physiology,Stanford University, 2Makine Mühendisliği Bölümü,Stanford University, 3Biyomühendislik Bölümü,Stanford University, 4Group of Neurophotonics and Mechanical Systems Biology,The Institute of Photonic Sciences (ICFO)

Özet

Automatically generated

New tools for mechanobiology research are needed to understand how mechanical stress activates biochemical pathways and elicits biological responses. Here, we showcase a new method for selective mechanical stimulation of immobilized animals with a microfluidic trap allowing high-resolution imaging of cellular responses.

Explore More Videos

MikroakışkanlarMechanical StimulationHigh-resolution ImagingC. ElegansMechanobiologySensory BiologyPressure ActuatorsGCaMPRFPFluorescence MicroscopyM9 BufferPeristaltic PumpPDMS

Read Article

Cite this Article

Fehlauer, H., Nekimken, A. L., Kim, A. A., Pruitt, B. L., Goodman, M. B., Krieg, M. Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans. J. Vis. Exp. (132), e56530, doi:10.3791/56530 (2018).

Download .ris file

Copy

Share Video

.

Copy