Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of <em>C. elegans</em>

Holger Fehlauer; Adam L. Nekimken; Anna A. Kim; Beth L. Pruitt; Miriam B. Goodman; Michael Krieg

doi:10.3791/56530

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神経科学

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Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

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神経科学

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JoVE Journal 神経科学

Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

9,607 Views

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10:39 min

•

February 19, 2018

DOI:

10.3791/56530-v

DOI:

10.3791/56530-v

•

10:39 min

February 19, 2018

•

3 Views

Holger Fehlauer*¹, Adam L. Nekimken*^1,2, Anna A. Kim^1,2, Beth L. Pruitt^1,2,3, Miriam B. Goodman^1,2, Michael Krieg⁴

¹Department of Molecular and Cellular Physiology,Stanford University, ²機械工学科,Stanford University, ³生物工学科,Stanford University, ⁴Group of Neurophotonics and Mechanical Systems Biology,The Institute of Photonic Sciences (ICFO)

概要

New tools for mechanobiology research are needed to understand how mechanical stress activates biochemical pathways and elicits biological responses. Here, we showcase a new method for selective mechanical stimulation of immobilized animals with a microfluidic trap allowing high-resolution imaging of cellular responses.

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Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

概要

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