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Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans
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神经科学
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JoVE 杂志 神经科学
Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

9,607 Views

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10:39 min

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February 19, 2018

DOI:

10.3791/56530-v

DOI:
10.3791/56530-v

•

10:39 min
February 19, 2018

•

3 Views
, , , , ,
1Department of Molecular and Cellular Physiology,Stanford University, 2Department of Mechanical Engineering,Stanford University, 3Department of Bioengineering,Stanford University, 4Group of Neurophotonics and Mechanical Systems Biology,The Institute of Photonic Sciences (ICFO)

Summary

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New tools for mechanobiology research are needed to understand how mechanical stress activates biochemical pathways and elicits biological responses. Here, we showcase a new method for selective mechanical stimulation of immobilized animals with a microfluidic trap allowing high-resolution imaging of cellular responses.

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Cite this Article

Fehlauer, H., Nekimken, A. L., Kim, A. A., Pruitt, B. L., Goodman, M. B., Krieg, M. Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans. J. Vis. Exp. (132), e56530, doi:10.3791/56530 (2018).

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