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用小鼠模型对精子发生细胞学分析的精管壁球技术
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Developmental Biology
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JoVE Journal Developmental Biology
A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
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用小鼠模型对精子发生细胞学分析的精管壁球技术

DOI:
10.3791/56453-v

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09:40 min

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February 06, 2018

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, ,
1Department of Biochemistry and Molecular Biology,Johns Hopkins Bloomberg School of Public Health

Chapters

  • 00:04Title
  • 01:12Dissection and Preparation of Mouse Seminiferous Tubules
  • 03:16Squashing and Labeling Prepared Seminiferous Tubules
  • 05:47Analysis and Imaging Tubule Squash Preparations
  • 07:03Results: Visualization of Meiotic Progression Using the Tubule Squash Preparation
  • 08:35Conclusion

Summary

Automatic Translation

Automatic Translation

这个小管壁技术的目的是快速评估发育中鼠精的细胞学特征, 同时保持细胞完整性。这种方法允许研究精子发生的所有阶段, 并可以很容易地实现与其他生物化学和分子生物学方法的研究小鼠减数分裂。

Tags

Seminiferous TubuleCytological AnalysisSpermatogenesisMouse ModelSpermatogoniaSpermatocytesSpermatidsMeiosisMale Reproductive BiologySpermatogonial Stem Cell DifferentiationMeiotic Chromosome SegregationPost-meiotic DifferentiationCellular IntegrityInfertilityMammalian SystemsSquash TechniquePBSFixative Lysis SolutionPoly-L-lysine Coated Glass Slide

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