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A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
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A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
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February 06, 2018
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Summary
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The goal of this tubule squash technique is to rapidly assess cytological features of developing mouse spermatocytes while preserving cellular integrity. This method allows for the study of all stages of spermatogenesis, and can be easily implemented alongside other biochemical and molecular biological approaches for the study of mouse meiosis.
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Seminiferous TubuleCytological AnalysisSpermatogenesisMouse ModelSpermatogoniaSpermatocytesSpermatidsMeiosisMale Reproductive BiologySpermatogonial Stem Cell DifferentiationMeiotic Chromosome SegregationPost-meiotic DifferentiationCellular IntegrityInfertilityMammalian SystemsSquash TechniquePBSFixative Lysis SolutionPoly-L-lysine Coated Glass Slide
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Wellard, S. R., Hopkins, J., Jordan, P. W. A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model. J. Vis. Exp. (132), e56453, doi:10.3791/56453 (2018).
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